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From where is the expected pattern obtained in restriction digestion?

Answer and explanation

Correct answer: From restriction map or sequence analysis

The expected restriction-digestion pattern is predicted from the restriction-enzyme recognition sites in DNA and the fragment sizes between those sites. This information is obtained from a restriction map or DNA sequence analysis, so option A is correct. Enzyme concentration may affect whether digestion is complete, but it does not determine the predicted fragment pattern. Exam tip: use the number and positions of cut sites on a map to predict fragment number and size.

Related tags

Restriction DigestionRestriction MapSequence AnalysisDna FragmentsAgarose Gel ElectrophoresisCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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