What is not added in a negative control of restriction digest reaction?Restriction digest reaction में negative control में क्या नहीं डाला जाता?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: restriction enzyme / Restriction enzymerestriction enzyme / Restriction enzyme
The governing concept is a no-enzyme negative control. To test whether DNA changes are caused by restriction digestion rather than handling, the control should contain the same DNA, buffer, water, and other relevant components as the experimental reaction, but it must omit the active restriction enzyme. The untreated control pattern can then be compared with the enzyme-treated sample on a gel. Therefore option A is correct. DNA is needed as the substrate and buffer maintains the required chemical conditions; water adjusts volume and concentration. Omitting these would make the comparison invalid. The purpose is not to create a reaction product, but to reveal the starting or uncut DNA pattern.यहाँ मुख्य अवधारणा no-enzyme negative control है। यह जाँचने के लिए कि DNA में आया परिवर्तन restriction digestion के कारण है या केवल handling के कारण, control में experimental tube के समान DNA, buffer, water और अन्य आवश्यक components रखे जाते हैं, लेकिन active restriction enzyme नहीं डाला जाता। फिर untreated control और enzyme-treated sample के gel patterns की तुलना की जाती है। इसलिए विकल्प A सही है। DNA substrate के रूप में आवश्यक है, buffer सही chemical conditions बनाए रखता है और water final volume तथा concentration समायोजित करता है। इन components को हटाने पर तुलना उचित नहीं रहेगी। Control का उद्देश्य कोई नया product बनाना नहीं, बल्कि uncut या प्रारंभिक DNA pattern दिखाना है।
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