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Why is sterile technique useful in restriction digestion?

Answer and explanation

Correct answer: To reduce the entry of contaminating microorganisms and nucleases that can damage DNA

Sterile technique involves clean equipment, nuclease-free water, and careful handling. It reduces the entry of external microorganisms and, especially, nucleases such as DNase, which can cause unwanted DNA degradation. Sterile handling does not change a restriction enzyme’s sequence specificity. Exam tip: In DNA-based experiments, contamination control and nuclease-free conditions primarily protect the DNA sample.

Related tags

Restriction DigestionSterile TechniqueNuclease ContaminationDna DegradationRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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