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Why can an adapter be used in restriction digestion based work?

Answer and explanation

Correct answer: To add a desired restriction end

The governing concept is adapter-mediated creation of a useful cloning end. An adapter is a short synthetic double-stranded DNA molecule designed with a desired sequence or end, often including a restriction site or an overhang compatible with a vector. When attached to a DNA fragment, it can provide an end that the original fragment did not possess, allowing directional or otherwise planned ligation into a vector. Thus option B is correct. An adapter is not used to destroy the restriction enzyme, convert DNA into lipid, or dry an electrophoresis gel. Its role is to modify the fragment’s termini so the next cloning step becomes possible or more efficient.

Related tags

AdapterRestriction EndCloning StrategyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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