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What can happen if two different restriction enzymes create the same overhang?

Answer and explanation

Correct answer: The ends can ligate

The governing concept is compatibility of restriction-generated DNA ends. Two enzymes may recognize different nucleotide sequences but leave identical or compatible single-stranded overhangs. Because the exposed bases can pair with one another, fragments produced by those enzymes can anneal and then be joined covalently by DNA ligase. Therefore option A is correct. Compatibility does not mean that the two enzymes are identical, and the junction may no longer recreate either original recognition site, depending on the sequences and cut positions. DNA does not disappear, an enzyme does not turn into a protein—it already is a protein—and gel staining is unrelated to end compatibility.

Related tags

Compatible EndsRestriction EnzymesLigationCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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