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What can happen if an insert joins in the wrong reading frame?

Answer and explanation

Correct answer: A protein with an incorrect amino acid sequence or a nonfunctional protein may be produced

During translation, mRNA codons are read in groups of three nucleotides. If an insert is not joined in the correct reading frame, the downstream codons may change, leading to incorrect amino acids; it may also introduce a premature stop codon. Therefore, an incorrect or nonfunctional protein may be produced. Option A would be expected only when the insert is in the correct frame. Exam tip: In protein-expression questions, check both the orientation and the reading frame of the insert.

Related tags

Reading FrameInsert CloningProtein ExpressionRecombinant DnaTranslationCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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