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What is the direct connection between restriction digest and ligation?

Answer and explanation

Correct answer: Restriction digest creates compatible DNA ends, which DNA ligase joins

A restriction endonuclease cuts DNA at specific recognition sequences and produces sticky or blunt ends. When the vector and desired DNA fragment have compatible ends, DNA ligase forms phosphodiester bonds to join them. Option B reverses the functions of the enzymes. Exam tip: remember that restriction enzymes cut, whereas ligase joins.

Related tags

Recombinant Dna TechnologyRestriction DigestionDna LigaseCompatible EndsSticky EndsDna CuttingCutting Of DnaProcesses Of Recombinant Dna Technology

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