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What can be the result of too little enzyme in restriction digestion?

Answer and explanation

Correct answer: Partial digestion

If the amount of restriction enzyme is too low, it may not cut all recognition sites in the DNA. Some sites therefore remain uncut, resulting in partial digestion. Star activity is generally associated with conditions such as an unsuitable buffer, excess enzyme, or prolonged incubation, not too little enzyme. Exam tip: Link insufficient enzyme or insufficient incubation time with incomplete/partial digestion.

Related tags

Restriction DigestionRestriction EnzymePartial DigestionDna CuttingRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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