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What is one likely reason for smear in restriction digest?

Answer and explanation

Correct answer: Degraded DNA

Degraded DNA contains a mixture of many irregular fragment sizes. During gel electrophoresis, these fragments may appear as a diffuse smear rather than sharp bands. Perfectly intact DNA would usually give a clear band according to its size, so it is not the likely cause. Exam tip: when a smear is observed, check DNA quality, nuclease contamination, and sample handling.

Related tags

Restriction DigestionDna DegradationGel ElectrophoresisDna QualityRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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