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What is generally obtained when a circular plasmid has two cut sites?

Answer and explanation

Correct answer: Two DNA fragments

When a circular plasmid DNA is cut at two different sites, it is divided into two DNA fragments. Therefore, two DNA fragments is correct. A single cut would generally produce one linear DNA fragment, making option B the closest but incorrect distractor. Exam tip: for circular DNA, the number of fragments generally equals the number of cut sites.

Related tags

Recombinant Dna TechnologyPlasmid DnaRestriction DigestionDna CuttingCircular DnaRestriction EnzymesCutting Of DnaProcesses Of Recombinant Dna Technology

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