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Class 11BiotechnologyProcesses of Recombinant DNA TechnologyCutting of DNAEasy
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Why is cleanliness of DNA ends important before ligation after restriction cutting?

Answer and explanation

Correct answer: Contaminants can inhibit ligase activity

Salt ethanol phenol or enzymes can interfere with ligation. Clean DNA ends give better joining.

Related tags

Dna EndsLigationCleanupCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq
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10 more MCQs
  1. 1What are blunt ends?
  2. 2Where do restriction enzymes make cuts in DNA?
  3. 3What must be done before inserting a desired gene into a cloning vector?
  4. 4Which term best describes the action of a restriction enzyme?
  5. 5When are sticky ends in restriction-cut DNA useful?
  6. 6What can be done to a foreign gene before inserting it into a vector in cloning?
  7. 7Why are restriction-cut DNA fragments shown in gel electrophoresis?
  8. 8If plasmid and insert are cut with two different enzymes what can be a benefit?
  9. 9What problem may occur if the vector and insert have incompatible ends?
  10. 10Which enzymes are mainly used for cutting DNA?
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