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Subjects
Class 11BiotechnologyProcesses of Recombinant DNA TechnologyCutting of DNAEasy
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Why can cleanup be needed after alkaline phosphatase treatment?

Answer and explanation

Correct answer: To remove phosphatase that can affect ligation

Residual phosphatase can keep removing phosphate in ligation setup. Therefore cleanup is useful.

Related tags

Alkaline PhosphataseCleanupLigationCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq
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10 more MCQs
  1. 1What are blunt ends?
  2. 2Where do restriction enzymes make cuts in DNA?
  3. 3What must be done before inserting a desired gene into a cloning vector?
  4. 4Which term best describes the action of a restriction enzyme?
  5. 5When are sticky ends in restriction-cut DNA useful?
  6. 6What can be done to a foreign gene before inserting it into a vector in cloning?
  7. 7Why are restriction-cut DNA fragments shown in gel electrophoresis?
  8. 8If plasmid and insert are cut with two different enzymes what can be a benefit?
  9. 9What problem may occur if the vector and insert have incompatible ends?
  10. 10Which enzymes are mainly used for cutting DNA?
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