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Why can diagnostic digest be difficult without a restriction map?

Answer and explanation

Correct answer: The expected DNA fragment sizes and band pattern cannot be predicted

A restriction map shows the locations of restriction-enzyme cut sites. It allows prediction of the DNA fragment sizes and the band pattern expected after digestion on a gel. Without the map, it is difficult to compare the observed bands with an expected result. Enzyme activity, the negative charge of DNA, and agarose-gel preparation do not depend on a restriction map. Exam tip: In a diagnostic digest, a restriction map is mainly used to predict fragment sizes and band patterns.

Related tags

Diagnostic DigestRestriction MapRestriction EnzymesDna FragmentsGel ElectrophoresisRecombinant DnaCutting Of DnaProcesses Of Recombinant Dna Technology

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