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Why does restriction digestion not always directly confirm insert orientation?Restriction digestion से insert orientation directly क्यों हर बार confirm नहीं होती?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Sometimes band pattern can be same for orientationsकभी band pattern orientation में समान हो सकता है
Informative restriction sites are needed to confirm orientation. Otherwise sequencing or orientation PCR may be needed.Orientation confirm करने के लिए informative restriction sites चाहिए। Otherwise sequencing या orientation PCR जरूरी हो सकता है।
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