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How can different enzymes giving the same overhang be useful in cloning?

Answer and explanation

Correct answer: They can enable compatible ligation between DNA fragments

If two different restriction enzymes produce the same complementary sticky overhang, a vector cut by one enzyme can base-pair with a DNA fragment cut by the other. DNA ligase can then join these compatible ends to form recombinant DNA. The other options are incorrect because restriction enzymes cut at specific recognition sites rather than breaking all DNA bonds; they do not synthesise proteins or control gel migration. Exam tip: Ends with matching overhangs are called compatible ends, even when produced by different enzymes.

Related tags

Restriction EnzymesSticky EndsCompatible EndsDna LigationRecombinant DnaCloningCutting Of DnaProcesses Of Recombinant Dna Technology

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