Why are compatible sticky ends useful in restriction digestion?Restriction digestion में compatible sticky ends क्यों useful होते हैं?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: They can do complementary base pairingवे complementary base pairing कर सकते हैं
Sticky ends are short single-stranded overhangs produced when a restriction enzyme cuts DNA in a staggered way. If two DNA fragments have compatible sticky ends, the exposed bases can form temporary complementary base pairs. This pairing brings the fragment ends together in the correct alignment and makes joining more efficient.
Thus option A is correct. The pairing itself is not a permanent chemical bond. DNA ligase is needed afterward to seal the sugar-phosphate backbones by forming the final phosphodiester bonds. Compatible ends do not make protein, destroy the restriction enzyme, or melt an agarose gel. Their usefulness comes from base complementarity and the alignment it provides during recombinant-DNA construction.Sticky ends वे छोटे single-stranded overhangs हैं जो restriction enzyme द्वारा DNA को staggered तरीके से काटने पर बनते हैं। यदि दो DNA fragments के sticky ends compatible हों, तो उनके खुले हुए bases complementary base pairing कर सकते हैं। यह pairing दोनों fragments को सही alignment में पास लाती है और उन्हें जोड़ना अधिक आसान बनाती है।
इसलिए विकल्प A सही है। यह pairing स्वयं permanent chemical bond नहीं बनाती। इसके बाद DNA ligase sugar-phosphate backbones को phosphodiester bonds द्वारा seal करता है। Compatible ends protein नहीं बनाते, restriction enzyme को destroy नहीं करते और agarose gel को melt नहीं करते। उनका मुख्य लाभ complementary bases और उचित alignment से मिलता है।
Student feedback
Was this question useful?
👍 0 Helpful 👎 0 Not helpful
Yes 0% No 0%
0 responsesStudent Reviews
No published reviews yet.
