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Why can higher agarose percentage be useful for small DNA fragments?

Answer and explanation

Correct answer: It forms smaller pores and resolves small DNA fragments better

Increasing the agarose percentage makes the pores of the gel smaller. Small DNA fragments are separated more clearly according to their size in such a gel, giving better resolution. Option B is incorrect because a higher agarose concentration generally slows DNA migration rather than increasing its speed. Exam tip: remember that high-percentage agarose is preferred for small fragments, whereas low-percentage agarose is used for larger fragments.

Related tags

Agarose Gel ElectrophoresisDna Fragment SeparationSmall Dna FragmentsGel ResolutionRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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