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Why is gel loading dye added in restriction digestion analysis?

Answer and explanation

Correct answer: To help load the sample into wells and track DNA migration

Gel loading dye commonly contains glycerol or sucrose, which makes the sample denser so that it settles into the agarose-gel well. It also contains tracking dyes that help monitor the progress of DNA migration during electrophoresis. DNA is cut by a restriction enzyme, not by the loading dye. Exam tip: remember the two key roles of loading dye—easy loading and migration tracking.

Related tags

Restriction DigestionGel Loading DyeAgarose Gel ElectrophoresisDna MigrationRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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