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What is the risk of keeping DNA band on UV transilluminator for too long?

Answer and explanation

Correct answer: DNA damage

UV radiation from a transilluminator can damage DNA by causing lesions such as strand breaks or thymine dimers. Therefore, UV exposure should be kept as short as possible while cutting a gel band, especially if the DNA will be used later for ligation or cloning. A brighter band does not mean that the amount of DNA has increased. Exam tip: prolonged UV exposure is associated with DNA damage.

Related tags

Uv TransilluminatorDna DamageGel ElectrophoresisGel ExtractionRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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