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When can gel extraction be necessary?

Answer and explanation

Correct answer: To separate the desired DNA fragment from other DNA fragments

In gel extraction, the desired DNA band is excised from an agarose gel and the DNA is purified from it. Therefore, it is needed when digestion produces several DNA fragments and the correct insert must be isolated for cloning. It does not colour restriction enzymes or freeze buffers. Exam tip: remember that gel extraction purifies a specific DNA fragment from a gel band.

Related tags

Gel ExtractionDna FragmentAgarose GelInsert PurificationRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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