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Why are all restriction enzymes not heat-inactivated?

Answer and explanation

Correct answer: Each restriction enzyme has different heat stability

Restriction enzymes are proteins, and their structures and heat stabilities differ. Some are denatured and inactivated at a suitable high temperature, whereas others are relatively heat-stable; therefore, heat treatment cannot inactivate every restriction enzyme. Option C is incorrect because excessive heat usually disrupts enzyme structure rather than universally increasing activity. Exam tip: Check the recommended inactivation temperature and incubation time for a particular enzyme.

Related tags

Restriction EnzymesHeat InactivationEnzyme StabilityRecombinant DnaDna CuttingCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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