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Subjects
Class 11BiotechnologyProcesses of Recombinant DNA TechnologyCutting of DNAEasy
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If a restriction enzyme is methylation-sensitive what should be considered in cloning plan?

Answer and explanation

Correct answer: DNA source and methylation status

Methylated DNA can block enzyme cutting. Therefore a suitable host strain or alternative enzyme can be chosen.

Related tags

Methylation SensitiveDna SourceAlternative EnzymeCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq
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10 more MCQs
  1. 1What are blunt ends?
  2. 2Where do restriction enzymes make cuts in DNA?
  3. 3What must be done before inserting a desired gene into a cloning vector?
  4. 4Which term best describes the action of a restriction enzyme?
  5. 5When are sticky ends in restriction-cut DNA useful?
  6. 6What can be done to a foreign gene before inserting it into a vector in cloning?
  7. 7Why are restriction-cut DNA fragments shown in gel electrophoresis?
  8. 8If plasmid and insert are cut with two different enzymes what can be a benefit?
  9. 9What problem may occur if the vector and insert have incompatible ends?
  10. 10Which enzymes are mainly used for cutting DNA?
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