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What is the best use of MCS in a vector?

Answer and explanation

Correct answer: Facilitating cloning by providing multiple unique restriction sites

An MCS (multiple cloning site) is a short region of a vector containing several different, usually unique, restriction enzyme recognition sites. It allows the vector and the desired DNA fragment to be cut with a suitable enzyme and joined conveniently for cloning. Restriction enzymes are not stored in the vector; they are separately used in the laboratory to cut DNA. Exam tip: MCS is also called a polylinker.

Related tags

Multiple Cloning SiteCloning VectorRestriction SitesRecombinant Dna TechnologyDna InsertionCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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