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Why is cut fragment and vector ratio important in restriction digestion based cloning?

Answer and explanation

Correct answer: It affects ligation efficiency

Suitable amounts of insert and vector help ligation success. Wrong ratio can give empty vector or low clones.

Related tags

Insert Vector RatioLigation EfficiencyCloningCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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