What is the main difference between restriction digestion and PCR?

Correct answer: Restriction digestion cuts DNA at specific recognition sites, whereas PCR makes many copies of a target DNA sequence.

In restriction digestion, a restriction endonuclease recognizes specific DNA sequences and cuts the DNA to produce fragments. In contrast, PCR uses primers and DNA polymerase to make many copies of a selected DNA segment. Option B reverses the functions of the two processes. Exam tip: associate restriction enzymes with cutting and PCR with amplification.