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What problem can occur if a restriction site is also inside the insert?

Answer and explanation

Correct answer: Insert can cut into unwanted fragments

If the chosen enzyme cuts inside the insert the desired gene can break. Check sequence before enzyme choice.

Related tags

Internal Restriction SiteInsert DamageEnzyme ChoiceCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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