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What is the immediate purpose of DNA cutting in making recombinant DNA?

Answer and explanation

Correct answer: To create compatible ends on the insert DNA and vector DNA

In recombinant DNA technology, a restriction endonuclease cuts the insert DNA and vector DNA at specific sites to produce compatible ends that can base-pair. Formation of permanent phosphodiester bonds between these ends is done by DNA ligase, so option C describes the later ligation step, not cutting. Exam tip: cutting produces compatible ends; ligation permanently joins DNA fragments.

Related tags

Recombinant DnaRestriction EndonucleaseDna CuttingCompatible EndsVector DnaDna LigaseCutting Of DnaProcesses Of Recombinant Dna Technology

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