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Why is restriction enzyme kept on ice?

Answer and explanation

Correct answer: To preserve enzyme activity and stability

Restriction enzymes are proteins whose structure or activity may be reduced at higher temperatures. Keeping them on ice helps maintain enzyme stability and activity until the digestion reaction is started. DNA amplification is done by PCR, not by a restriction enzyme. Exam tip: Enzymes used for cutting DNA are generally kept cold during setup.

Related tags

Restriction EnzymeDna DigestionEnzyme StabilityRecombinant Dna TechnologyLaboratory TechniqueCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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