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What effect can incorrect pH have in a restriction enzyme reaction?

Answer and explanation

Correct answer: Enzyme activity can decrease

A restriction enzyme works most effectively within a specific pH range. An incorrect pH can affect the enzyme's structure or active site, reducing or even preventing DNA cleavage. Incorrect pH does not automatically increase DNA purity. Exam tip: remember that the recommended buffer provides the correct pH and conditions for restriction digestion.

Related tags

Restriction EnzymesRestriction DigestionPhEnzyme ActivityDna CuttingRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna Technology

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