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What can be an example of a negative control in restriction digestion?

Answer and explanation

Correct answer: DNA without restriction enzyme

In a negative control, the same DNA is kept without adding the restriction enzyme. Therefore, the DNA remains uncut and gives the band pattern of uncut DNA on the gel. Comparing this pattern with the enzyme-treated sample shows whether the change in bands is due to restriction digestion. A tube containing only enzyme and buffer but no DNA may help check contamination, but it is not the uncut-DNA control. Exam tip: the usual negative control for restriction digestion is “DNA without enzyme.”

Related tags

Restriction DigestionNegative ControlUncut DnaRestriction EnzymeGel ElectrophoresisCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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