What does vector linearization after restriction digestion mean?Restriction digestion के बाद vector linearization का क्या अर्थ है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Cutting a circular vector so that it becomes linearCutting a circular vector so that it becomes linear
The governing concept is preparation of a cloning vector for insertion. Many plasmid vectors are circular double-stranded DNA molecules. When a restriction enzyme cuts the plasmid at a selected recognition site, the circular molecule is opened and becomes a single linear DNA molecule with two ends. These ends can then interact with a suitably cut insert and be joined by DNA ligase. Option A is correct because linearization specifically means opening the circular vector by cleavage. The process does not translate DNA into protein, transcribe it into RNA, or ferment it into ethanol; therefore options B, C, and D describe unrelated transformations.इसका मुख्य सिद्धान्त cloning vector को insert जोड़ने के लिए तैयार करना है। कई plasmid vectors circular double-stranded DNA molecules होते हैं। जब restriction enzyme plasmid की चुनी हुई recognition site पर cut करता है, तो circular molecule खुलकर एक linear DNA molecule बन जाता है और उसके दो ends बनते हैं। ये ends उपयुक्त enzyme से काटे गए insert के साथ जुड़ सकते हैं तथा DNA ligase द्वारा स्थायी रूप से जोड़े जा सकते हैं। इसलिए विकल्प A सही है, क्योंकि linearization का अर्थ circular vector को काटकर linear बनाना है। इससे DNA protein, RNA या ethanol में परिवर्तित नहीं होता; अतः B, C और D असंबंधित हैं।
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