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Class 11BiotechnologyProcesses of Recombinant DNA TechnologyCutting of DNAEasy
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Why can restriction digest fail due to DNA methylation?

Answer and explanation

Correct answer: Methylation can block enzyme recognition

Some enzymes do not cut methylated recognition sites. Methylation sensitivity should be checked in enzyme choice.

Related tags

Dna MethylationEnzyme RecognitionDigest FailureCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq
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10 more MCQs
  1. 1What are blunt ends?
  2. 2Where do restriction enzymes make cuts in DNA?
  3. 3What must be done before inserting a desired gene into a cloning vector?
  4. 4Which term best describes the action of a restriction enzyme?
  5. 5When are sticky ends in restriction-cut DNA useful?
  6. 6What can be done to a foreign gene before inserting it into a vector in cloning?
  7. 7Why are restriction-cut DNA fragments shown in gel electrophoresis?
  8. 8If plasmid and insert are cut with two different enzymes what can be a benefit?
  9. 9What problem may occur if the vector and insert have incompatible ends?
  10. 10Which enzymes are mainly used for cutting DNA?
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