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What can happen by repeated freeze-thaw of restriction enzyme?

Answer and explanation

Correct answer: Enzyme activity can decrease

A restriction enzyme is a protein. Repeated freezing and thawing can destabilize its structure and reduce its ability to cut DNA. Doubling the amount of DNA occurs through amplification methods such as PCR, not through freeze-thaw cycles. Exam tip: restriction enzymes are commonly stored in small aliquots to avoid repeated freeze-thawing.

Related tags

Restriction EnzymesFreeze ThawEnzyme StabilityDna CuttingRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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