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How can compatible sticky ends be produced by different enzymes?

Answer and explanation

Correct answer: If they produce same or compatible overhangs

The direct answer is option A: different enzymes can produce compatible sticky ends when their overhangs are the same or can base-pair with each other. A restriction enzyme cuts DNA and may leave a short single-stranded overhang called a sticky end. Two ends are compatible if their exposed bases can pair correctly, allowing DNA ligase to join them. The recognition sequences used by the enzymes may be different, but the important point is the sequence and direction of the overhang. Option A is correct because same or compatible overhangs can anneal and then be sealed by ligase. Option B is wrong: DNA cannot have no bases and still provide a meaningful sticky end. Option C is wrong because the mere presence of protein does not make DNA ends compatible. Option D is irrelevant: melting a gel does not create matching DNA overhangs. The memory cue is: different cutters may still be useful if their sticky ends match.

Related tags

Compatible Sticky EndsOverhangsLigation

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