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What is the main role of cutting DNA in recombinant DNA technology?

Answer and explanation

Correct answer: To prepare DNA fragments and compatible vector ends

The governing concept is restriction-based DNA cutting during recombinant DNA construction. A restriction enzyme recognizes a particular sequence and cleaves the DNA, producing a desired insert fragment and an opened, or linearized, vector. If the same or compatible enzymes are used, the ends of the insert and vector can pair by complementary base pairing, allowing DNA ligase to join them in the next step. Option A is correct because cutting creates the physical DNA pieces and compatible ends required for assembly. Option B concerns protein digestion, not DNA cloning. Options C and D describe biologically impossible conversions and are unrelated to recombinant DNA construction.

Related tags

Dna CuttingRestriction EnzymeVector InsertCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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