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Class 11BiotechnologyProcesses of Recombinant DNA TechnologyCutting of DNAEasy
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What effect can DNA methylation have in restriction digestion?

Answer and explanation

Correct answer: It can affect enzyme recognition or cutting

Some restriction enzymes do not cut methylated sites. Therefore methylation status can change digestion results.

Related tags

Dna MethylationRestriction EnzymeCuttingCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq
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10 more MCQs
  1. 1What are blunt ends?
  2. 2Where do restriction enzymes make cuts in DNA?
  3. 3What must be done before inserting a desired gene into a cloning vector?
  4. 4Which term best describes the action of a restriction enzyme?
  5. 5When are sticky ends in restriction-cut DNA useful?
  6. 6What can be done to a foreign gene before inserting it into a vector in cloning?
  7. 7Why are restriction-cut DNA fragments shown in gel electrophoresis?
  8. 8If plasmid and insert are cut with two different enzymes what can be a benefit?
  9. 9What problem may occur if the vector and insert have incompatible ends?
  10. 10Which enzymes are mainly used for cutting DNA?
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