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Why is a DNA ladder loaded to view restriction digestion results?

Answer and explanation

Correct answer: To estimate the sizes of DNA fragments

A DNA ladder contains DNA fragments of known base-pair lengths. During gel electrophoresis, the positions of sample bands are compared with ladder bands to estimate the sizes of DNA fragments produced by restriction digestion. The restriction enzyme cuts DNA; the ladder does not activate it. Exam tip: smaller DNA fragments generally migrate farther through an agarose gel.

Related tags

Dna LadderRestriction DigestionGel ElectrophoresisDna Fragment SizeRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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