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Why is temperature controlled in a restriction enzyme reaction?

Answer and explanation

Correct answer: To maintain proper enzyme activity

A restriction enzyme works most efficiently at its optimum temperature, so the reaction temperature is controlled. At an incorrect temperature, enzyme activity may decrease or the enzyme may denature, leading to incomplete or inefficient DNA cutting. Melting gel is not the purpose of restriction digestion. Exam tip: Many restriction enzymes have an optimum temperature of 37°C, but always check the enzyme-specific conditions.

Related tags

Restriction EnzymesRestriction DigestionEnzyme ActivityOptimal TemperatureDna CuttingRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna Technology

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