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Why is specificity of a restriction enzyme important?

Answer and explanation

Correct answer: Because it cuts at a specific recognition sequence in DNA to produce the required fragments

A restriction enzyme recognizes a particular DNA recognition sequence and cuts at or near that site. This produces predictable DNA fragments, which are needed to isolate a desired gene and insert it into a vector during cloning. Option C describes the action of DNA ligase, not a restriction enzyme. Exam tip: restriction enzymes cut DNA, whereas ligases join DNA fragments.

Related tags

Restriction EnzymesDna SpecificityRecognition SequenceDna CuttingRecombinant Dna TechnologyCloningCutting Of DnaProcesses Of Recombinant Dna Technology

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