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What type of ends are generally produced by EcoRI cutting?

Answer and explanation

Correct answer: Sticky ends

EcoRI is a restriction endonuclease that cuts the two DNA strands in a staggered manner rather than directly opposite each other. This produces short single-stranded overhangs called sticky ends. These overhangs can pair with complementary bases and make ligation by DNA ligase easier. Blunt ends do not have such overhangs. Exam tip: remember that staggered cuts produce sticky ends, whereas straight cuts produce blunt ends.

Related tags

EcoriRestriction EndonucleaseSticky EndsDna CuttingRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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