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Biotechnology

Ligase Enzymes

In this Class 11 Biotechnology topic from Principles of Biotechnology, students learn how ligase enzymes join DNA fragments by forming phosphodiester bonds between adjacent nucleotides. The topic explains the role of DNA ligase in repairing breaks, sealing fragments with compatible sticky or blunt ends, and constructing recombinant DNA molecules. Students also connect ligase activity with gene cloning, vector construction, and the basic steps of laboratory-based genetic engineering.

Hard · Level 1 · 1 questions

Practice questions

01E. coli DNA ligase is commonly associated with which cofactor?

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Answer and explanation

Correct answer: A. NAD⁺ / NAD plus

The relevant biochemical distinction is that the usual E. coli DNA ligase is NAD⁺-dependent, whereas bacteriophage T4 DNA ligase is commonly described as ATP-dependent. NAD⁺ participates in activation of the DNA-adenylate intermediate required for ligation, allowing the enzyme to seal a nick by forming a phosphodiester bond. Therefore option A, NAD⁺, is correct. ATP is an important cofactor for several other ligases, including T4 ligase, but “ATP only” is not the standard association for the E. coli enzyme in this school-level comparison. Chlorophyll is a photosynthetic pigment and starch is a storage carbohydrate, so neither is a ligase cofactor.

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Ligase Enzymes

Practice questions

01E. coli DNA ligase is commonly associated with which cofactor?E. coli DNA लाइगेज सामान्यतः किस सहकारक से जुड़ा होता है?

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01E. coli DNA ligase is commonly associated with which cofactor?