किस step के बिना cut insert और vector केवल कमजोर रूप से जुड़े रह सकते हैं?
Without which step can cut insert and vector remain only weakly associated?
Explanation opens after your attempt
A. ligation by DNA ligase
Concept
Sticky ends can make weak association by base pairing. DNA ligase creates a permanent phosphodiester bond.
Why this answer is correct
The correct answer is A. ligation by DNA ligase. Sticky ends can make weak association by base pairing. DNA ligase creates a permanent phosphodiester bond.
Exam Tip
Sticky ends base pairing से weak association बना सकते हैं। DNA ligase permanent phosphodiester bond बनाता है।
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