एक practical में ligase क्यों last में add किया जा सकता है?
Why can ligase be added near the last step in a practical?
Explanation opens after your attempt
Correct Answer
A. क्योंकि पहले DNA ends तैयार किए जाते हैं फिर उन्हें जोड़ा जाता हैBecause DNA ends are prepared first and then joined
Concept
In cloning compatible ends of vector and insert are made first. Then ligase joins them.
Why this answer is correct
The correct answer is A. क्योंकि पहले DNA ends तैयार किए जाते हैं फिर उन्हें जोड़ा जाता है / Because DNA ends are prepared first and then joined. In cloning compatible ends of vector and insert are made first. Then ligase joins them.
Exam Tip
Cloning में पहले vector और insert के compatible ends बनाए जाते हैं। फिर ligase उनका joining करता है।
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