यदि insert और vector के सिरों में 5 prime phosphate नहीं है तो ligation पर क्या असर होगा?
If insert and vector ends lack 5 prime phosphate what happens to ligation?
Explanation opens after your attempt
Correct Answer
A. लाइगेशन प्रभावित हो सकती हैLigation may be affected
Concept
5 prime phosphate is needed to form a phosphodiester bond. Its absence can reduce ligation efficiency.
Why this answer is correct
The correct answer is A. लाइगेशन प्रभावित हो सकती है / Ligation may be affected. 5 prime phosphate is needed to form a phosphodiester bond. Its absence can reduce ligation efficiency.
Exam Tip
5 prime phosphate phosphodiester bond बनाने के लिए जरूरी होता है। इसकी कमी ligation efficiency घटा सकती है।
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