Alkaline phosphatase cloning में self-ligation कम करने में कैसे मदद कर सकता है?
How can alkaline phosphatase help reduce self-ligation in cloning?
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Correct Answer
A. कटे vector से पाँच प्राइम फॉस्फेट हटाकरBy removing 5 prime phosphate from cut vector
Concept
Removing 5 prime phosphate from vector ends can reduce self-ligation. In exams link phosphate removal with ligation control.
Why this answer is correct
The correct answer is A. कटे vector से पाँच प्राइम फॉस्फेट हटाकर / By removing 5 prime phosphate from cut vector. Removing 5 prime phosphate from vector ends can reduce self-ligation. In exams link phosphate removal with ligation control.
Exam Tip
Vector ends से पाँच प्राइम फॉस्फेट हटाने पर self-ligation घट सकती है। परीक्षा में phosphate removal को ligation control से जोड़ें।
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