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Biotechnology Competent Host MCQ Questions for Class 11 Science

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01 Practice Topic Genetic Engineering 200 MCQs 02 Practice Topic Bioprocess Engineering 300 MCQs 03 Practice Topic Restriction Enzymes 345 MCQs 04 Practice Topic Ligase Enzymes 290 MCQs 05 Practice Topic Cloning Vectors 295 MCQs

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Question 81/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 31

Cloning host में restriction activity कम होना क्यों उपयोगी है?

Why is reduced restriction activity useful in a cloning host?

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D. Incoming plasmid DNA protect रहता हैIncoming plasmid DNA remains protected

Explanation

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Reduced restriction activity foreign plasmid degradation घटाती है। इससे transformation success बढ़ सकती है। / Reduced restriction activity decreases degradation of foreign plasmid. This can improve transformation success.

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Question 82/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 31

Plasmid methylation status क्यों important हो सकता है?

Why can plasmid methylation status matter?

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A. Host restriction enzymes DNA recognition को प्रभावित कर सकते हैंHost restriction enzymes can be affected by DNA recognition

Explanation

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Methylation कुछ restriction systems से DNA को बचा सकती है या digestion pattern बदल सकती है। यह host system पर निर्भर करता है। / Methylation can protect DNA from some restriction systems or change digestion pattern. It depends on the host system.

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Question 83/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 31

Transformation में sterile spreader क्यों उपयोग किया जाता है?

Why is a sterile spreader used in transformation?

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B. Contamination रोकने के लिएTo prevent contamination

Explanation

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Sterilization unwanted microbes को plate पर आने से रोकती है। यह सही colony interpretation के लिए जरूरी है। / Sterilization prevents unwanted microbes from entering the plate. It is necessary for correct colony interpretation.

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Question 84/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 31

Plate पर बहुत अधिक colonies होने से क्या समस्या हो सकती है?

What problem can too many colonies on a plate cause?

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C. Single colonies isolate करना कठिन होगाIt will be difficult to isolate single colonies

Explanation

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बहुत dense growth में अलग colonies चुनना कठिन होता है। Proper dilution clone isolation में मदद करता है। / In very dense growth picking separate colonies is difficult. Proper dilution helps clone isolation.

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Question 85/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 31

बहुत कम colonies मिलने का एक कारण क्या हो सकता है?

What can be one reason for getting very few colonies?

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D. Low transformation efficiency

Explanation

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Few colonies low competence low DNA quality या wrong selection का संकेत हो सकती हैं। यह troubleshooting point है। / Few colonies can indicate low competence poor DNA quality or wrong selection. This is a troubleshooting point.

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Question 86/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 31

Positive control plate पर no colonies क्या बता सकता है?

What can no colonies on a positive control plate indicate?

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A. Cells competent नहीं रहीं या method failedCells are not competent or method failed

Explanation

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Positive control failure method या cells की problem दिखाता है। यह troubleshooting में important है। / Failure of positive control shows a problem with the method or cells. It is important in troubleshooting.

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Question 87/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 31

Negative control पर colonies आना क्या बता सकता है?

What can colonies on a negative control indicate?

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B. Contamination या selection failureContamination or selection failure

Explanation

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Negative control में plasmid नहीं होता। Colonies contamination या antibiotic selection problem दिखा सकती हैं। / Negative control has no plasmid. Colonies can show contamination or antibiotic selection problem.

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Question 88/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 31

Plasmid DNA entry के बाद सबसे पहले क्या जरूरी है?

After plasmid DNA entry what is important first?

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C. Cells survive करें और marker express करेंCells survive and express marker

Explanation

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Transformation के बाद cells को recover होना और resistance marker express करना चाहिए। तभी वे selection plate पर बढ़ेंगी। / After transformation cells should recover and express resistance marker. Then they can grow on selection plates.

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Question 89/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 31

Expression host में inducer कब use हो सकता है?

When can an inducer be used in an expression host?

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D. Gene expression शुरू कराने के लिएTo start gene expression

Explanation

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Inducer controlled promoter को activate कर सकता है। यह recombinant protein expression में common idea है। / An inducer can activate a controlled promoter. This is a common idea in recombinant protein expression.

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Question 90/445 Easy Biotechnology Principles of Biotechnology Competent Host Class 11 Level 31

IPTG किस concept से जुड़ा हो सकता है?

IPTG can be linked with which concept?

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A. Induction of gene expression

Explanation

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IPTG lac-based expression systems में inducer की तरह use हो सकता है। इसे expression host context में याद रखें। / IPTG can be used as an inducer in lac-based expression systems. Remember it in expression host context.

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