किस scenario में blunt-end ligation sticky-end ligation से कठिन हो सकती है?
In which scenario can blunt-end ligation be harder than sticky-end ligation?
Explanation opens after your attempt
Correct Answer
A. जब complementary overhang मौजूद न होंWhen complementary overhangs are absent
Concept
Blunt ends lack overhangs so base pairing support is absent. This can make ligation comparatively harder.
Why this answer is correct
The correct answer is A. जब complementary overhang मौजूद न हों / When complementary overhangs are absent. Blunt ends lack overhangs so base pairing support is absent. This can make ligation comparatively harder.
Exam Tip
Blunt ends में overhang नहीं होते इसलिए base pairing सहायता नहीं मिलती। इससे ligation तुलनात्मक रूप से कठिन हो सकती है।
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