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Ligase Enzymes

Class 11 Biotechnology के इस tag से जुड़े questions। हर question के साथ chapter, topic, level और difficulty दी गई है।

BiotechnologyWhich option correctly relates ligase and Mg ion?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhat can happen if proper buffer is absent in ligase reaction?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyIf a ligase mixture lacks ATP and the enzyme is T4 ligase what may happen?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhich option gives a correct reason for ligase reaction failure?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhy is ligase enzyme kept in cold condition?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhich factor can affect ligation efficiency?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhat is the risk if vector is high and insert is low in ligase reaction?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhy is proper vector and insert ratio important in ligase reaction?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhat may be missing in a self-ligated vector?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhat is the benefit of directional cloning in ligation?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyIn directional cloning what does ligase join?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyIf vector is dephosphorylated what may be important for insert ligation?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhich treatment can reduce vector self-ligation?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhat can be the effect of removing 5 prime phosphate in a ligase reaction?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyLigase forms a covalent bond between what?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhat happens on the lagging strand in absence of DNA ligase?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhich option correctly relates ligase and Okazaki fragments?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhich type of bond does DNA ligase repair or create?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyE coli DNA ligase can be distinguished by which cofactor?Class 11Principles of BiotechnologyLigase EnzymesEasyBiotechnologyWhich option correctly gives energy requirement of T4 DNA ligase?Class 11Principles of BiotechnologyLigase EnzymesEasy

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