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Sticky ends are produced by what?

Answer and explanation

Correct answer: By staggered cutting

Sticky ends are produced when a restriction endonuclease cuts the two strands of double-stranded DNA at different positions, creating a staggered break. The resulting DNA fragments have short single-stranded overhangs. These overhangs contain bases that can pair with a complementary sequence on another fragment, making it easier to align the fragments before DNA ligase seals the sugar-phosphate backbone. Therefore option A is correct. Direct heating may denature DNA but does not create the specific, enzyme-defined overhangs used in recombinant DNA work. Reading RNA and folding proteins are biological processes unrelated to restriction cutting. The governing concept is staggered restriction-enzyme cleavage followed by complementary base pairing and ligation.

Related tags

Sticky EndsRestriction EnzymesRecombinant DnaStaggered CuttingPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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