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How is restriction enzyme unit useful in reaction planning?

Answer and explanation

Correct answer: In estimating enzyme activity amount

An enzyme unit is a standardized measure of catalytic activity under a defined assay condition, commonly related to the amount of enzyme needed to produce a specified extent of DNA cleavage in a specified time. Reaction planning uses this information to select enough units for the quantity and type of DNA present, while respecting the supplier’s recommendations and the buffer conditions. For example, a larger DNA mass or a more complex substrate may require proportionally more activity, although the exact requirement depends on the enzyme and protocol. Units do not describe smell, count gel pores, or directly read a DNA sequence. They estimate functional activity, making option B correct.

Related tags

Enzyme UnitReaction PlanningDna AmountRestriction EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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